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puller  (Sutter Instrument Company)


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    Sutter Instrument Company puller
    Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 632 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flaming/P-97+Flaming%2FBrown+Type+Micropipette+Puller/pmc13626651-243-14-16
    Average 96 stars, based on 632 article reviews
    puller - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Patch Clamp:

    Article Title: Decoding subtype development and function in human pluripotent stem cell-derived midbrain dopaminergic neurons
    Article Snippet: .. For whole-cell patch-clamp studies, borosilicate glass pipettes (G150F-4, Warner Instruments, CT) with a tip resistance of 3-4 MΩ were pulled on a P-97 Flaming-Brown micropipette puller (Sutter Instruments, CA) and filled with (in mM): 115 K-gluconate, 20 KCl, 10 HEPES, 2 MgCl2, 2 ATP-Mg, 2 ATP-Na2 and 0.3 GTP-Na, (pH 7.25, ∼280 mOsm). .. Neurons were visualized under a 40x water immersion objective using Olympus BX51W1 microscope (Olympus), and recording were performed with an MultiClamp 700B amplifier (Molecular Devices, CA) and digitized at 10 kHz with ITC-18 (HEKA Instruments Inc, NY).

    Glass:

    Article Title: Decoding subtype development and function in human pluripotent stem cell-derived midbrain dopaminergic neurons
    Article Snippet: .. For whole-cell patch-clamp studies, borosilicate glass pipettes (G150F-4, Warner Instruments, CT) with a tip resistance of 3-4 MΩ were pulled on a P-97 Flaming-Brown micropipette puller (Sutter Instruments, CA) and filled with (in mM): 115 K-gluconate, 20 KCl, 10 HEPES, 2 MgCl2, 2 ATP-Mg, 2 ATP-Na2 and 0.3 GTP-Na, (pH 7.25, ∼280 mOsm). .. Neurons were visualized under a 40x water immersion objective using Olympus BX51W1 microscope (Olympus), and recording were performed with an MultiClamp 700B amplifier (Molecular Devices, CA) and digitized at 10 kHz with ITC-18 (HEKA Instruments Inc, NY).

    Article Title: Loss of sirtuin SIRT1 promotes aggressiveness of colon adenocarcinoma by modulating voltage-gated sodium channel activity
    Article Snippet: Nikon Eclipse TE300 microscope was used to visualize cells. .. Patch pipettes were double pulled from borosilicate glass to a resistance of 5–7 MΩ using Flaming/Brown micropipette puller (Sutter instrument co., USA) and filled with intrapipette solution containing (in mM): 130 KCl, 15 NaCl, 0.37 CaCl2, 1 MgCl2, 1 Mg-ATP, 1 EGTA, 10 HEPES, pH adjusted to 7.2. ..

    Article Title: P2X7 receptor antagonism potentiates seizure-suppressive effects of anti-seizure medications in human resected epileptic brain tissue
    Article Snippet: .. Field recordings were obtained using borosilicate glass capillaries pulled with a micropipette puller (Sutter Instrument Co. Model P-97 Flaming) filled with ACSF with a resistance of 3-10 MΩ positioned in grey matter of the slice. .. The glass electrodes were connected to an NL100AK headstage, and the signals were amplified using an NL104 pre-amplifier (Digitimer, Hertfordshire, UK), band-pass filtered at 0.5 Hz to 500 Hz using NL125 Filter module (Digitimer, Hertfordshire, UK), and digitized using a CED Micro 1401 (Cambridge Electronic Design Ltd., Cambridge, UK) interfaced with a Windows 10 PC.

    Article Title: Zinc is a key regulator of the sperm-specific K + channel (Slo3) function
    Article Snippet: .. Recording pipettes were made with borosilicate glass (BF-150-86-10; Sutter Instruments, CA, USA) using a puller (P-97; Sutter Instruments). .. The recordings were conducted using an Axopatch 200B amplifier (Molecular Devices, CA, USA) and sampled at 5 kHz using a Digidata 1550A system (Molecular Devices) with pCLAMP 10.5 software (Molecular Devices).

    other:

    Article Title: Mesocortical dopamine hypofunction and prefrontal plasticity deficits in the cuprizone model.
    Article Snippet: Recording pipettes were prepared from borosilicate capillaries containing an internal filament using a P-97 Flaming Brown micropipette puller (Sutter Instruments, Novato, CA, USA).

    Transferring:

    Article Title: The HCN4 channel modulates external tufted cell burst firing
    Article Snippet: .. 0.75 mm; Sutter Instrument, Novato, CA) in a horizontal pipette puller P-97 (Sutter Instrument, Novato, CA, USA). ..



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